Immune issues - start here guide to this section!

[Baby Mama]

Many thanks for the prompt response

[FertilityFriends]

[debsmc1]

Hello All

hope you don't mind me adding into this thread... just looking for some advice.. had one MC summer 2013 and failed first IVF in Oct 14 after having had laprascopy for removal of chocolate cyst and mild endometritis...

just did the Serum menstrual blood tests and found ureaplasma infection which I am about to start taking course of antibiotics for (and other half) ... I was also planning to do at my cycle in Feb to get a womb biopsy with Dr Siobhan Quenby to check my NK killer cells ... I think the results can though take 6 weeks to come back and keen to start next IVF cycle in April time...

should I wait and get the NK killer cells test done now and wait for results rather than go through another iVF and maybe fail if there is something with the NK Killer cells...

time running out for me as nearly 34 

any advice appreciated

[agate]

possibly if you have ureaplasma you'd expect your NK to come back raised so maybe there isn't much point in doing until you've finished antibs.... but then I wouldn't bother do the biopsy at all if you aren't going to find out the results before you do tx so you can take them into account in your ivf protocol?

[Monicap28]

Hi all,
Sorry for jumping in but i ve just had my immune results come through. The first complete ones ive ever had done .
Can anyone, agate ? please help me interpret these results further or has anyone had any success with similar results.
Ive already been on two immune protocols  incl GCSF, intralipid & Pregni', ending in chemical preganancies so after seeing these results, is there anything here that can give me hope or anything i can do further  ?

NK Assay Inhibition Panel

100% DQalpha Match
 
50:1         - 27.6 ( tbh i thought this would be higher due to the dqalpha match)
25:1         -22.5
12.5:1       - 15.6

IVIG 12.5 mg/ml, 50.1    - 15.4
IVIG 12.5 mg/ml, 25.1    – 8.0
Intralipid 1.5mg/ml 50:1  – 22.2
Intralipid 1.5mg/ml 25:1   – 15.3

% CD3 – 82.4
% CD19 – 10.4
%CD56 – 7.3
% of CD19 cells CD5+  - 1.9

TH1:TH2 intracellular cytokine ratios
 
TNF-a:IL-10 (CD3+CD4+)      26.8 (thought this would be over 30 due to dqalpha match)
IFN-g:IL-10 (CD3+CD4+)           13.7

Leukocyte Antibody Detection
 (Ive had 3 LIT's done in greece)
Flowcytometry           
[T-cells] IgM+               12.6
[T-cells] IgG+               57.2
[B-cells] IgM+              30.4
[B-cells] IgG+              20.7
 
Only 1 KIR activator found ie 2DS4

[agate]

So reduced immunoreg due to missing KIR.

27% killing power is v high so probably due to DQa. 

Lad i s higher than mine got after first 4 lits so thats a win in my book

[LuckyE]

Hi Agate

I have three tests back from Serum. Can you help out what they mean in terms as to what immunes I would be taking?

NK CYTOTOXICITY ASSAY – ANALYSIS REPORT
Effector to      Incubation time   NK Cytotoxicity   Typical range
Target Ratio      time (hr)      %         %

50:1         4         25.20          26–56
25:1                   14.90          15–39
12.5:1                9.47          12–26


The patient’s cytotoxicity level is marginally below the normal range at 50:1 and 25:1 ratios, and below at the 12.5:1 ratio

TH1:TH2 intracellular cytokine ratios
 
Ratios                              Typical range
(CD3+CD4+) IFNγ/IL–10   25,80    5,80 – 20,30
 (CD3+CD4+)ΤΝFα/IL–10  21,48      13,20 – 30,60
 
The results show that the (CD3+CD4+) IFNγ/IL–10 ratio is slightly above the normal range. Our control sample was within the typical range for both ratios.

The third test - all the figures were in range:

REPORT OF PERIPHERAL BLOOD LYMPHOCYTES
IMMUNOPHENOTYPIC ANALYSIS

Results : The natural killer (NK) cell concentration was found to be normal compared to the mean value ofgeneral population (80–170 cells/μl). Additionally, the percentage of natural killer (NK) cells was found to be normal (12%, Coulam et al 1995).

Thanks in advance

LuckyE

[agate]

Basically normal

[LuckyE]

Yay! so no steroids needed or blood thinners?

[LuckyE]

And thank you Agate for your prompt response. You are an angel.

[agate]

Low dose steroids possibly?